Plasmid_Backbone

Part:BBa_K4215000:Design

Designed by: Carmen Resnick   Group: iGEM22_UOregon   (2022-10-10)


pSEVA341


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3132
    Illegal SpeI site found at 3194
    Illegal PstI site found at 3144
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SpeI site found at 3194
    Illegal PstI site found at 3144
    Illegal NotI site found at 3162
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 3126
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3132
    Illegal SpeI site found at 3194
    Illegal PstI site found at 3144
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3132
    Illegal SpeI site found at 3194
    Illegal PstI site found at 3144
    Illegal NgoMIV site found at 819
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3220
    Illegal BsaI.rc site found at 3085


Design Notes

The plasmid is designed for expression of split assay proteins including beta lactamase so the bla antibiotic resistant gene had to be removed. The golden gate sites incorporate the start and stop codons into the sequence for ease of gene insertion.


Source

pSEVA was constructed from combining pSEVA1411 and replacing the bla gene with CAM to exchange the antibiotic resistances. CAM was extracted from plasmid SMT205.

References